Romanian Society of Pharmaceutical Sciences
 

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BIOCHEMICAL MECHANISMS OF OXIDATIVE STRESS IN ANIMALS EXPOSED TO HEXAVALENT CHROMIUM COMPOUNDS IN THE CASE OF ISONIAZID–RIFAMPICIN HEPATITIS

NATALIYA GARLITSKA 1*, LYUDMYLA FIRA 2, PETRO LYKHATSKYI 3, LARYSA BOYKO 1

1.Department of General Chemistry, Faculty of Pharmacy, I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine
2.Department of Pharmacy, Educational Scientific Institute of Postgraduate Education, I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine
3.Department of Medical Biochemistry, Faculty of Medicine, I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine

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We evaluated the effects of the combined administration of isoniazid, rifampicin and hexavalent chromium compounds for 7 and 14 days, in rats. Analysing the blood serum, it was observed a more significant accumulation of POL (lipid peroxidation products) at this level than in the liver, which may be a consequence of the damage of hepatocyte membranes and the incidence of toxic factors in blood. The maximum decrease of catalase activity in liver was recorded in the immature rats of the 3rd experimental group till the end of the experiment. The poisoning of rats by potassium dichromate on the background of induced hepatitis resulted in the most significant decrease of the content of reduced glutathione, on the 14th day of the study. The introduction of toxic substances in the animal organisms caused significant infringements and decreased levels of both enzymatic and non-enzymatic antioxidant defence systems, as evidenced by the strong correlation between catalase activity and the content of reduced glutathione. An indirect correlation was observed between the content of TBA-RP and catalase activity in the liver of animals, which were exposed to potassium dichromate (r = –0.86).