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A BOX-BEHNKEN DESIGN FOR OPTIMIZATION OF ULTRASOUND-ASSISTED EXTRACTION OF SINAPIC ACID FROM FRAGARIA ANANASSA

AJAZ AHMAD 1*, MOHAMMAD RAISH 2, NAUSHAD ALI 3, BILAL AHAMAD PARAY 4

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The present study focuses on optimizing the extraction process of sinapic acid from Fragaria ananassa using ultrasonic-assisted extraction (UAE). The extraction process study variables were: temperature in the range of 20 to 60°C, extraction time between 20 and 90 minutes and the solid-to-liquid ratio ranging from 10 to 30 mL/g. Response surface analysis determined that the most favourable conditions for sinapic acid extraction were at a temperature of 60°C, extraction time of 55 minutes and at a liquid-to-solid ratio of 30 mL/g. Under these conditions, the extraction yield of sinapic acid was measured at 23.56 mg/g. The experimental results closely aligned with the predicted values, affirming the reliability of the proposed model. Furthermore, UAE proved to be a highly effective method, exhibiting significantly reduced extraction time and producing higher levels of sinapic acid compared to traditional extraction techniques. HPLC analysis confirmed the presence of substantial amounts of sinapic acid in Fragaria ananassa. In conclusion, the optimized UAE method, in conjunction with HPLC-UV analysis, presents a valuable approach for the efficient extraction and estimation of sinapic acid from Fragaria ananassa. These results have the potential to greatly benefit industrial applications that need for extremely high extraction yields of these organic molecules.