COMPARTMENT‑SPECIFIC ADIPOKINE AND INFLAMMATORY SIGNATURES IN BREAST AND PANCREATIC CANCER: A CLINICAL‑ TRANSLATIONAL PILOT STUDY
MARA MARDARE 1,2 , FLAVIA ULTIMESCU 1,2*, ANDREI BOGDAN VĂCĂRAȘU 1,2, IRINA BONDOC 1,2, ALINA PUȘCAȘU 1,2, DIANA IULIANA STANCIU 3, DENISA CRĂCIUN 3 , ARIANA HUDIȚĂ 3, BIANCA GĂLĂȚEANU 3, GERALD GHEORGHE FILIP 4, MIHAIL CEAUȘU 1,5 , MIRCEA-BOGDAN MĂCIUCEANU-ZĂRNESCU 1,6 , GEORGE-TRAIAN- ALEXANDRU BURCEA-DRAGOMIROIU 1, OCTAV GINGHINĂ 1,2
1 “Carol Davila” University of Medicine and Pharmacy, Bucharest, Romania
2 “Prof. dr. Al. Trestioreanu” Oncologic Institute of Bucharest, Bucharest, Romania
3 Faculty of Biology, University of Bucharest, Bucharest, Romania
4 Ponderas Academic Hospital, Bucharest, Romania
5 “C.I. Parhon” National Institute of Endocrinology, Bucharest, Romania
6 Bucharest Emergency Clinical Hospital, Bucharest, Romania
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This study aimed to investigate the profiles of adipokines and cytokines found in urine, peritumoral adipose tissue, and subcu- taneous adipose tissue from patients with breast and pancreatic cancer. We also wanted to understand their clinical and trans- lational significance in relation to body mass index (BMI), systemic inflammation, and tumor markers. To achieve this, we measured levels of adiponectin, adipsin, RPB-4, MCP-1, IL-1β, IP-10, IL-10, IL-8, leptin, IL-6, IFN-γ, resistin, and TNF-α in urine and the two types of adipose tissue using a LEGENDplex multiplex assay for flow cytometry. We observed notable differences across biological compartments for all mediators we examined, with urinary concentrations significantly higher than those in adipose tissue. The urinary leptin‑to‑adiponectin (L/A) ratio showed a significant positive correlation with body mass index and emerged as a more robust indicator of metabolic imbalance than either leptin or adiponectin alone. Tumor‑infiltrating T‑cell analysis, available for a small subset of patients, revealed marked inter‑individual variability without permitting statisti- cal inference.
