Romanian Society of Pharmaceutical Sciences
 

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THE EFFECTS OF ELICITOR AND ABIOTIC STRESSOR MOLECULES ON PHENOLIC COMPOSITION OF IN VITRO CALLUS CULTURES OF ELECAMPANE (INULA HELENIUM L.)

PEYAMI BATTAL 1*, GÖKHAN AKÇURA 2,3, MUSA TÜRKER 3, ABDULLAH DALAR 4,5

1 Department of Medical Services and Techniques, Vocational School of Health Services, Gazi University, 06830, Ankara, Türkiye
2 Faculty of Medicine, Bahçeşehir University, 36734, Istanbul, Türkiye
3 Department of Bioengineering, Faculty of Chemistry and Metallurgy, Yildiz Technical University, 32410, Istanbul, Türkiye
4 Mardin Artuklu University Herbarium (MARIUM), Mardin Artuklu University, Mardin 47060, Türkiye
5 Department of Pharmaceutical Botany, Faculty of Pharmacy, Van Yuzuncu Yil University, Van 65090, Türkiye

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This study focused on the effects of various stressors and elicitors on phenolic metabolism of in vitro callus cultures of Elecampane. 1 mg/L 2,4-Dichlorophenoxyacetic acid (2,4-D) + 0,3 mg/L Kinetin (KN) formulation was detected as the most proper application for callus production. Quercetin was synthesized as the dominant phenolic compound of callus cultures. The highest values of total phenolics were obtained through the combination of 6-Benzylaminopurine + 1-Naphthaleneacetic acid (BAP+NAA) in the plant growth regulators (PGR) group, ½ reduced Murashige and Skoog medium (MS) in the stressor group, and both abscisic acid and melatonin in the elicitor group. The application of various PGRs and elicitors enhanced the level of quercetin at pronounced levels, but not in rutin. Chlorogenic acid increased under silver nitrate and ¼ reduced MS stressors and melatonin and abscisic acid elicitors. These findings suggest that the combination of 2,4-D + KN, reduced MS, abscisic acid, and melatonin conditions stimulated the synthesis of phenolics through the biosynthetic pathway by increasing the stress resistance of Elecampane.