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A NEW APPROACH FOR EVALUATION OF ULTRAFILTRATION METHOD FOR DETERMINATION OF RABEPRAZOLE FROM HUMAN PLASMA BY RP-HPLC-DAD

MERVE NENNİ ¹*, MUSTAFA ÇELEBİER ², İNCILAY SÜSLÜ 2

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Ultrafiltration-based sample preparation (UFBSP) is a common method in the food and pharmaceutical industries. Sample preparation is the first stage in pharmaceutical analysis. The purpose of this study was to investigate the presence of rabeprazole (Rab) in human plasma samples by using UFBSP. The findings of UFBSP in biological analyses were compared with those of conventional methods so that both the benefits and the drawbacks of this technology could be assessed. The chemical that causes precipitation was utilized before the UFBSP. The examination was performed on an HPLC-UV with C18 column. The mobile phase: acetonitrile:water, flow rate: 1.0 mL min-1; injection volume: 20 μL; wavelength: 230 nm. The bioanalytical method was proven effective by using it on samples of Rab that had been added to commercial human plasma samples. This was carried out in compliance with the guidelines that had been laid down by the FDA. LOD and LOQ were 0.0201 and 0.067 μg/mL, respectively. The approach had excellent determination coefficients (R2 > 0.999) and was linear over the range of 0.1 - 15.0 μg/mL in terms of concentration. The results of this analysis demonstrated that the matrix minimized the effect of the method offered for sample preparation. LOD and LOQ values were discovered to have relatively low, while it was discovered that the recovery values had comparatively high, coupled with good trueness and precision. The validation parameters of the suggested method were tested by putting RAB into plasma that had already been spiked. As a comparison method, a lot of the HPLC methods that have been written about were used.