Romanian Society of Pharmaceutical Sciences
 

« Back to Farmacia Journal 5/2023

DEVELOPMENT AND VALIDATION OF A NEW VISIBLE SPECTROPHOTOMETRIC METHOD FOR DETERMINATION OF FLUCONAZOLE IN THE PRESENCE OF SOME ESSENTIAL OILS

IOANA OLARIU 1#, ANA MARIA MUŢ 1, VICENŢIU VLAIA 2*, PETRU CRĂCIUN 3, ŞTEFANIA COLGIU 4, ZOLTAN SZABADAI 5, LAVINIA VLAIA 1, EUGEN GABRIEL TURCU 6#, DUMITRU LUPULIASA 7, GEORGETA CONEAC 1

1Department II – Pharmaceutical Technology, Formulation and Technology of Drugs Research Centre, “Victor Babeș” University of Medicine and Pharmacy, 2 Eftimie Murgu Square, 300041, Timișoara, Romania
2 Department II – Organic Chemistry, Formulation and Technology of Drugs Research Centre, “Victor Babeș” University of Medicine and Pharmacy, 2 Eftimie Murgu Square, 300041, Timișoara, Romania
3 Department of Drug Industry and Pharmaceutical Biotechnology, Faculty of Pharmacy, “Carol Davila” University of Medicine and Pharmacy, 6 Traian Vuia Street, 020956, Bucharest, Romania
4 PhD student, Doctoral School, “Victor Babeș” University of Medicine and Pharmacy, 2 Eftimie Murgu Square, 300041, Timișoara, Romania
5 National Institute for Research and Development in Electrochemistry and Condensed Matter, 114 Dr. Aurel Păunescu Podeanu Street, 300587 Timişoara, Romania
6 Department of Plastic Surgery, “Bagdasar-Arseni” Clinical Emergency Hospital, “Carol Davila” University of Medicine and Pharmacy, 12 Berceni Road, 041915, Bucharest, Romania
7 Department of Pharmaceutical Technology and Biopharmacy, Faculty of Pharmacy, “Carol Davila” University of Medicine and Pharmacy, 6 Traian Vuia Street, 020956, Bucharest, Romania

Download Full Article PDF

This study aimed to develop and validate a visible spectrophotometry analysis method for fluconazole quantification in the presence of some essential oils (cinnamon, oregano and clove essential oil), by forming a coloured complex with Cu2+ ion. For the spectrophotometric method validation the spectra of the fluconazole-Cu(II) complex in the presence of volatile oils were analysed and the wavelengths corresponding to the maximum absorbance values of the complex were determined. Evaluating the precision, linearity, accuracy and robustness of the elaborated visible spectrophotometric method, the obtained values of the specific parameters fell within the limits provided by the standards in force. Linearity was very good for the following fluconazole concentration ranges: 0.268 - 2.68 mg/mL for oregano essential oil, 0.2073 - 2.073 mg/mL for cinnamon essential oil and 0.2317 - 2.317 mg/mL in the case of clove essential oil. The proposed visible spectrophotometric method has been successfully applied for the assay of fluconazole in clove, cinnamon and oregano essential oils.