Romanian Society of Pharmaceutical Sciences
 

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UHPLC/MS ASSESSMENT OF POLYPHENOLIC ACIDS AND RADICAL SCAVENGING ACTIVITY OF SALVIA SPECIES FROM THE SOUTH-WEST OF ROMANIA FLORA

ROXANA MARIA BĂLĂŞOIU 1, ANDREI BIŢĂ 2*, ELENA CAMELIA STĂNCIULESCU 3, GABRIELA RĂU 4, CORNELIA BEJENARU 5, LUDOVIC EVERARD BEJENARU 2, CĂTĂLINA GABRIELA PISOSCHI 3

Faculty of Pharmacy, University of Medicine and Pharmacy, 2–4 Petru Rareş Street, 200349, Craiova, Romania
1 PhD Student, Doctoral School, University of Medicine and Pharmacy of Craiova
2 Department of Pharmacognosy,
3 Department of Pharmaceutical Biochemistry,
4 Department of Organic Chemistry, 5Department of Pharmaceutical Botany

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Five species of Salvia harvested from Oltenia region flora, Romania, were analysed for polyphenolic and flavonoid content and radical scavenging activity using UHPLC/MS, Folin-Ciocâlteu, and AlCl3 spectrophotometric methods, respectively, DPPH and ABTS assays. UHPLC profiling revealed the presence of two polyphenolic carboxylic acids (rosmarinic and caffeic) and two flavonoids (luteolin and isoquercitrin) in all Salvia extracts. Rosmarinic acid was found in the highest amount in all samples. The methanolic extract of S. nemorosa proved to have the best radical scavenging activity in the DPPH test, while S. verticillata had the smallest IC50 in the ABTS assay. This study revealed data regarding the phytochemical profiles of these five Salvia spp. in relation to their antioxidant properties evaluated through radical scavenging activity.