Romanian Society of Pharmaceutical Sciences
 

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POLYPHENOLIC PROFILE AND ANTI-INFLAMMATORY, ANALGESIC AND ANTIOXIDANT EFFECTS OF ETHANOLIC AND HYDRO-ETHANOLIC EXTRACTS OF HEINSIA CRINITA AFZ.G. TAYLOR AND TETRACERA ALNIFOLIA WILLD

LUCE SARRAH BOUMBA 1, ANAMARIA CRISTINA 2*, FREDY GELASE NSONDE NTANDOU 3, ILIOARA ONIGA 4, DANIELA BENEDEC 4, ANA-MARIA VLASE 5, OLIVIU VOSTINARU 2, ANGE ANTOINE ABENA 6, CRISTINA MOGOSAN 2

1Laboratory of Chemistry of Organic Biomolecules and Pharmacodynamics, Department of Pharmacopoeia and Traditional
Medicine, National Institute for Research in Health Sciences (IRSSA), Cité Scientifique, Brazzaville, Congo
2 Department of Pharmacology, Physiology and Physiopathology, Faculty of Pharmacy, “Iuliu Haţieganu” University of Medicine and Pharmacy, 8 Victor Babeș Street, 400012, Cluj-Napoca, Romania
3 Animal Physiology and Physiopathology Laboratory, Faculty of Science and Technology, Marien Ngouabi University, Brazzaville, BP 69, Congo
4 Department of Pharmacognosy, Faculty of Pharmacy, “Iuliu Haţieganu” University of Medicine and Pharmacy, 8 Victor Babeș Street, 400012, Cluj-Napoca, Romania
5 Department of Pharmaceutical Botany, Faculty of Pharmacy, “Iuliu Haţieganu” University of Medicine and Pharmacy, 8 Victor Babeș Street, 400012, Cluj-Napoca, Romania
6 Laboratory of Biochemistry and Pharmacology, Faculty of Health Sciences, Marien Ngouabi University, Brazzaville, B.P. 69, Congo

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The aim of this study was to determine the anti-inflammatory, analgesic and antioxidant effects and the polyphenols composition of Tetracera alnifolia Willd and Heinsia crinita Afz. G. Taylor extracts. The anti-inflammatory effect of the hydro-ethanolic and ethanolic extracts was evaluated by the carrageenan-induced rat paw oedema test and the analgesic effect by the Randall-Selitto test. Antioxidant activity of the extracts was evaluated spectrophotometrically using the DPPH radical scavenging assay and the polyphenolic profile, by an HPLC-MS method. All extracts of H. crinita and T. alnifolia (500 mg/kg) showed significant anti-inflammatory and analgesic effects. The extract of T. alnifolia has shown an antioxidant effect greater than that of H. crinita. Gallic acid (33.31 µg/mL) and rutin (34.70 µg/mL) are the major polyphenolic compounds of T. alnifolia and H. crinita, respectively. This study justifies the use in the traditional medicine of T. alnifolia and H. crinita. The obtained results are promising for the use of these extract in the management of inflammatory diseases.