Romanian Society of Pharmaceutical Sciences
 

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RESEARCH REGARDING THE EFFECT OF LEPTIN UPON THE RATIO OF CERTAIN LYMPHOCYTE POPULATIONS IN RAT

MARIAN GHIȚĂ 1, RĂZVAN BOTEZATU 2, CRISTIN COMAN 3, VLAD VUȚĂ 4*, GABRIEL GÂJÂILĂ 1, ALINA CRENGUȚA NICOLAE 5, CRISTINA MANUELA DRĂGOI 5, GABRIEL COTOR 1

1University of Agronomic Sciences and Veterinary Medicine, 59 Mărăşti Boulevard, 011464, Bucharest, Romania 2Centrovet Veterinary Center, 52-54 Pascal Aristide Street, 031443, Bucharest, Romania
3 “Cantacuzino” National Medico-Military for Research and Development Institute, 103 Splaiul Independenței, 050096, Bucharest, Romania
4 Institute for Diagnosis and Animal Health, 63 Dr. Staicovici Street, 050557, Bucharest, Romania
5 “Carol Davila” University of Medicine and Pharmacy, Faculty of Pharmacy, Biochemistry Department, 020956, Bucharest, Romania

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The aim of the present research was to investigate the effects of exogenous leptin on the ratio of T and B lymphocytes populations in order to assess the theory regarding the implication of this hormone in the stimulation of the immune system. Rats included in the experimental group have been injected with leptin for 14 days. Blood samples from the experimental and control group were withdrawn on the 14th and 21st day after the leptin administration. Further on there were assessed different lymphocyte populations using the direct immunofluorescence technique: TCD3, TCD4, TCD8, and BCD45. The results revealed that after 14 days from the last inoculation, leptin induces stimulant effects on all the assessed lymphocyte populations. These effects were statistically insignificant (p > 0.05). Nevertheless, on the 21st day after the last inoculation, the stimulant effects were statistically significant (p < 0.01) for the lymphocytes: TCD3 (with 47.21%), TCD4 (with 39.68%) and TCD8 (with 50.43%). In addition, on the 21st day after the last inoculation, we found also a statistically significant stimulatory effect (p < 0.01) upon BCD45 lymphocytes (with 40.83%). However, this effect was weaker compared to the one manifested upon T lymphocytes because the responsiveness of B lymphocytes to leptin’s action was proved to be lower than the one for T lymphocytes. Based on our results, we can assert that leptin induces an amplification of the effectors proportion involved in the cellular mediated immune response.