Romanian Society of Pharmaceutical Sciences
 

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EXPERIMENTAL PHARMACOLOGICAL MODEL OF DIABETES INDUCTION WITH ALOXAN IN RAT

SIMONA NEGREŞ1, CORNEL CHIRIŢĂ1*, ELENA MOROŞAN2, ANDREEA LETIŢIA ARSENE3

1Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, University of Medicine and Pharmacy “Carol Davila”, Bucharest, Romania
2 Department of Clinical Laboratory and Nutritional Hygiene, Faculty of Pharmacy, University of Medicine and Pharmacy “Carol Davila”, Bucharest, Romania
3 Department of Biochemistry, Faculty of Pharmacy, University of Medicine and Pharmacy “Carol Davila”, Bucharest

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An experimental pharmacological model of diabetes induced with aloxan in white, male and female Wistar rats was designed. A collectivity of 312 animals was used, with an initial mean weight of 225.1 ± 31.48 g-bw and basal mean glycaemia of 103.0 ± 34.22 mg/dL. Diabetes was induced by administering intraperitoneally a dose of 130 mg/kg-bw alloxan. Experimental results showed that, in the tested collectivity, 66.34% of animals became diabetic, with a mean glycaemic value of 450.4 ± 129.7 mg/dL. For the diabetic group, decreases in the enzymatic activity were registered for glucose-6-phosphate dehydrogenase and hexokinase, accompanied by increases in the activity of glucose-6- phosphatase, as evidentiated in the experimental model of streptozotocin-induced diabetes. The alloxanic diabetes induced significant alterations of lipidic profiles in rat compared to the non-diabetic group, as follows: total cholesterol increased by 51.19%, plasma triglycerids increased by 50.30% and LDL-cholesterol increased by 59.46%. The designed pharmacological model can assist further in determining the antidiabetic potential of newly synthesized compounds or plant extracts.