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ZINC AND PIOGLITAZONE EFFECTS ON OVARIES AND ENDOMETRIUM IN DIABETES

CRISTINA GALES¹, CARMEN ZAMFIR¹, BOGDAN STOICA², MIHAI NECHIFOR^3*

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The effects of pioglitazone and zinc on ovary and endometrium of female non-pregnant rats during experimental-induced diabetes mellitus were tested. Adult female non-pregnant rats were divided into five groups. Group 1 served as control group receiving no substance. Group 2 received streptozotocin (STZ) 60 mg/kg bw i.p. (single dose). Groups 3, 4, and 5 received the same dose of STZ but also ZnCl2 (5 mg/kg bw/day i.p.), pioglitazone (5 mg/kg bw/day p.o.), and ZnCl2 (5 mg/kg bw/day i.p.) + pioglitazone (5 mg/kg bw/day p.o.), respectively, for 8 weeks. The plasma levels of glucose and the total antioxidant capacity were determined before STZ administration and at the end of the experiment. After 8 weeks, all animal were killed after anaesthesia and the ovaries and uterus from each animal were examined using optical microscopy and a program for morphometric. Zinc and pioglitazone both significantly reduced the number of atretic follicles. The endometrium thickness was reduced by STZ and improved by pioglitazone (13.53 ± 0.05 μm STZ group vs. 17.72 ± 0.34 μm in STZ + pioglitazone group p < 0.01). Pioglitazone and zinc reduced both the endometrial and ovarian damages in STZ – induced diabetes nonpregnant female rats.