THYMUS VULGARIS EXTRACT FORMULATED AS CYCLODEXTRIN COMPLEXES: SYNTHESIS, CHARACTERIZATION, ANTIOXIDANT ACTIVITY AND IN VITRO CYTOTOXICITY ASSESSMENT
IOANA TUȚĂ-SAS 1#, MARIA PROKS 1,2#, VIRGIL PĂUNESCU 1, IULIA PÎNZARU 2*, IOAN SAS 1, DORINA CORICOVAC 2, ALINA MOACĂ 2, CRISTINA DEHELEAN 2
1.Faculty of Medicine, “Victor Babeş” University of Medicine and Pharmacy Timișoara, 2 Eftimie Murgu Square, 300041 Timişoara, Romania
2.Faculty of Pharmacy, “Victor Babeş” University of Medicine and Pharmacy Timișoara, 2 Eftimie Murgu Square, 300041 Timişoara, Romania
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Thymus species are important sources of bioactive compounds but considering their low stability and solubility in aqueous solutions, the pharmaceutical utilization is limited. A method to overcome these drawbacks was proposed in the current study, by encapsulating thyme total hydro-alcoholic extract (TE) in β- and γ- CD (cyclodextrins). It was assessed the antioxidant activity (AOA) in time (DPPH assay) and the in vitro cytotoxicity on MCF-7 and MDA-MB-231 breast cancer cells, using the Alamar blue test (cells viability), scratch assay technique (cells migration and proliferation) and Annexin V-FITC assay (cells apoptosis). The TE and its CD complexes showed a significant AOA over a longer time interval indicating a controlled release of the active compounds. In vitro tests indicated a significant cytotoxic activity of both CD complexes as compared to TE, even at a low concentration (10 µg/mL), the most pronounced activity being recorded for TE γ-CD inclusion complex on MDA-MB-231 cells (32% viable cells at 100 µg/mL).