Romanian Society of Pharmaceutical Sciences

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THE EFFECTS OF HISTAMINE ON THE CONTROL OF CORNEAL NEOVASCULAR MOTILITY IN RATS

DANIELA BIANCA DAMIAN 1, ISABEL GHIȚĂ 1*, RAZVAN DANIEL IONESCU 2, ION FULGA 1

1.Departament of Pharmacology and Pharmacotherapy, Faculty of Medicine, “Carol Davila” University of Medicine and Pharmacy, Bucharest, Romania
2.Ophtalmic Emergency Clinical Hospital, Bucharest, Romania

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The aim of this study was to evaluate the existence of a possible histaminergic control in the corneal neovessels, in 45-dayold rat pups, on the model of neovascularization performed with ketamine. The experiments were performed on 45-day-old Wistar rats in which, starting with day 15, the experimental model of neovascularization was obtained by means of 5 repeated injections of ketamine at a dose of 150 mg/kg body weight. Recordings were made for each eye that developed neovascularization by examining 6 eyes for each experiment. Visualization of corneal neovascularization was performed using a Nikon stereomicroscope, model SMZ 1270, connected to a Mshot video camera. The total magnification was 400X. The diameter of the neovessel was measured at a chosen point, the same point/points for each recording made to that eye, over a period of 630 seconds, at different times. The parameters followed were the variations of the vascular calibre, the data obtained being analysed with Microsoft Office Excel. The results showed that there are histaminergic receptors in the corneal neovessels because histamine had a vasodilating effect. It has also been found that there are both H1-type and H2-type histaminergic receptors at this level, as both ranitidine and olopatadine have blocked the vasodilating effect of histamine. As neither ranitidine nor olopatadine had any effects when administered alone, it leads us to conclude that there is no histaminergic tonic control in the corneal neovessels.