THE EFFECT OF PLGA-COLLAGEN I PATCH ON INGUINAL HERNIA
FANGJIE ZHANG, GUODONG GAO *, PING WANG, JING YE, YONGGANG HUANG, HAO WU
Department of Hernia and Abdominal Wall Surgery, Hangzhou First People’s Hospital, Hangzhou, 310006, China
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Different approaches are used for the treatment of inguinal hernia, and patches play a central role. This study aimed to investigate the efficacy of a new patch with poly(lactic-co-glycolic acid) (PLGA) - Collagen I, on a murine model of inguinal hernia. 30 rats with induced abdominal wall defect were divided into 2 groups, an experimental group and a control group, 15 animals per group. The experimental group was treated with PLGA-Collagen I patch implanted with bone marrow mesenchymal stem cells (BMSCs) and the control group was treated with cell-free PLGA-Collagen I patch. After implantation each group was divided in 3 subgroups, 5 animals per subgroup intended to evaluate the changes at 1, 2 and 6 months. The pro-inflammatory factors IL-6 (interleukin -6) and IL-10 (interleukin -10) were investigated at 7, 14 and 28 days after implantation. The peritoneal inflammatory reactions were evaluated by identifying the macrophage surface antigen modifications in tissue. The regeneration of the tissue in the patch and the degradation state of the material was evaluated by haematoxylin-eosin (HE) staining. SEM was used to observe the integrity of peritoneum and the angiogenesis was detected by immunohistochemistry. After a week, the macrophages of both groups were significantly increased; after one month, the inflammatory reaction gradually subsided and the number of macrophages decreased. One month after surgery, there was no swelling in the abdominal wall of the experimental group and the control group. The PLGA-Collagen I patch of BMSCs can promote cell infiltration, angiogenesis and tissue regeneration. The degradation rate of PLGA increased. In sixth months, the BMSCs PLGA-Collagen I was almost completely degraded compared with cell free PLGA-Collagen I patches, in which the degradation was slower. PLGA-Collagen I tissue engineering materials planted with BMSCs can promote the repair of abdominal injury and can be a good candidate for the treatment of abdominal wall defects.