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THE CORRELATION BETWEEN DIFFERENT DOSES OF GLUTAMINE ON THE APOPTOSIS OF LYMPHOCYTES IN A MURINE MODEL OF SEPSIS

RAN LIU ^1#, XIANKE LI ^1#, KAI YU ^1*, YONG WANG ²

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The aim of this study was to investigate the effects of glutamine in different doses on the apoptosis of lymphocytes in septic mice. 100 healthy mice were randomly divided into a control group (A), a sepsis model group (B), a high-dose glutamine sepsis group (C, 0.75 mg/kgbw), a medium-dose glutamine sepsis group (D, 0.5 mg/kgbw) and a low-dose glutamine sepsis group (E, 0.35 mg/kgbw), 20 animals per group. Mice from B, C, D and E groups underwent cecal ligation and puncture (CLP), and then they were transformed into sepsis models. 24 hours after injection, the respiration, heart rate and rectal temperature of the mice were recorded. The thymus and spleen specimens were taken and fixed. The apoptosis of lymphocytes was detected by means of in suit end-labelling. Compared to the mice in group A, the mice in group B, C, D and E had lower rectal temperature, increased respiratory frequency and increased heart rate, satisfying the diagnostic criteria for animal models of Systemic Inflammatory Response Syndrome (SIRS). There were few apoptotic thymus and liver cells in group A, while cell apoptosis was obvious in the other four groups, suggesting a significant difference compared to group A (p < 0.05). Comparing the groups of different doses of glutamine, the number of apoptotic cells in groups C, D and E was significantly lesser than that in group B (p < 0.05). Glutamine in different doses had a large impact on the apoptosis of lymphocytes. The supplementation with glutamine can enhance the anti-apoptosis mechanism of the organism.