Romanian Society of Pharmaceutical Sciences
 

« Back to Farmacia Journal 1/2022

STANDARDIZATION OF ZIZIPHORA CLINOPODIOIDES LAM. CULTIVATED AND WILD GROWING IN THE SOUTH-CAUCASIAN FLORA

ULIKHANYAN GRETA 1,2*, TSATURYAN AVETIS 3, DUMANYAN KARINE 1, ALTUNYAN ASTGHIK 1, BEGLARYAN MARGARITA 4, HOVSEPYAN MAYA 5, CHICHOYAN NAIRA 1

1Yerevan State Medical University after M. Heratsi, Department of Pharmacognosy, Yerevan, Armenia,
2 Yerevan State Medical University after M. Heratsi, Department of Medical Physics, Yerevan, Armenia,
3 Scientific and Production Center «Armbiotechnology» National Academy of Sciences, RA, Yerevan, Armenia,
4 Yerevan State Medical University after M. Heratsi, Department of Pharmaceutical Management, Yerevan, Armenia, 5Yerevan State Medical University after M. Heratsi, Department of Medical Biology, Yerevan, Armenia

Download Full Article PDF

The aim of the current study was the qualitative and quantitative analysis of the extracts obtained from Ziziphora clinopodioides by HPLC and spectrophotometric methods. By the HPLC assay there were confirmed the qualitative-quantitative phenolic compositions of the substances in ethanol extracts; apigenin and verbascoside previously identified by thin layer chromatography. The study is using a modified spectrophotometric method for the characterization of the total amount of flavonoids with respect to 7-methyl sudahitin. HPLC analysis showed that the apigenin content ranged from 0.0024 to 0.01 mg/mL and the verbascoside-ranged from 0.114 to 0.504 mg/mL in Ziziphora dry extracts. It was shown that the plant Ziziphora accumulated a significant quantity of flavonoids of the total amount, 2.57% up to 4.18%. The current study indicated that the raw material of wild growing Ziziphora in floras of South-Caucasian region, cultivated in soil and in hydroponics conditions could represent a source of flavonoids, the quantity of which varies not only due to natural climatic conditions, but also due to the growing conditions.