Romanian Society of Pharmaceutical Sciences
 

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SIMULTANEOUS DETERMINATION OF ISONIAZID AND RIFAMPICIN BY UV SPECTROPHOTOMETRY

MARIANA TILINCA1, GABRIEL HANCU2*, ELEONORA MIRCIA3, DIANA IRIMINESCU2, AURA RUSU2, ROBERT ALEXANDRU VLAD2, ENIKŐ BARABÁS4

1.Cell and Molecular Biology, Faculty of Medicine, University of Medicine and Pharmacy Târgu Mureș, Romania
2 .Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Medicine and Pharmacy Târgu Mureș, Romania
3 .Department of Organic Chemistry, Faculty of Pharmacy, University of Medicine and Pharmacy Târgu Mureș, Romania
4 .Department of Botany and Cell Biology, Faculty of Pharmacy, University of Medicine and Pharmacy Târgu Mureş, Romania

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One of the most effective antituberculosis treatments is the combination between isoniazid and rifampicin. Two alternative UV spectrophotometric methods were developed for the simultaneous determination of isoniazid and rifampicin in mixture, by employing simultaneous equation method and first-derivative spectrophotometry respectively. The determinations were performed in methanol. In the simultaneous equation method, the absorbtion maxima of the two drugs, 263 nm and 338 nm, were selected for estimation of isoniazid and rifampicin respectively. The second method was based on a first-derivative spectrophotometric method involving the determination of the analytes at their respective zero crossing point, the determinations were made at 263 nm for rifampicin and 290 nm for isoniazid respectively. Both substances obeyed Lambert-Beer’s law in a concentration range between 5 - 50 µg/mL. The analytical performances of the proposed methods were verified regarding their accuracy, linearity, precision, selectivity and sensitivity according to ICH guidelines. The proposed methods can be successfully applied in routine laboratory analysis for the determination of isoniazid and rifampicin in combined dosage forms.