RESVERATROL SUPPRESSED LPS-INDUCED COX-2 VIA miR-146a-5p INHIBITION IN Raw246.7 CELLS
TRONG TUAN DAO1#, DO THI HA1#, TRAN THI HIEN2,3*, BUI THI BINH2, TRAN THI PHUONG4, PHUNG THANH LONG1,6, NGUYEN BICH THU1, DO THI NGUYET QUE5, NGUYEN MINH KHOI1, LE VIET DUNG1, TRAN MINH NGOC1
1.National Institute of Medicinal Materials, 3B Quangtrung, Hanoi, Vietnam
2.Department of Pharmacy, Thai Binh Medical and Pharmacy University, Vietnam
3.Faculty of Medicine, Lund University, Sweden
4.College of Pharmacy, Chonnam National University, South of Korea
5.Hanoi University of Pharmacy, 13-15 Le Thanh Tong, Hanoi, Vietnam
6.Dai Nam University, Vietnam
Download Full Article PDF
Trans-resveratrol (Res) is a well-known natural stilbene frequently found in grapes which have been reported to possess antioxidant, anti-cancer activities and inhibited COX-2 expression. MicroRNAs (miRNAs) are short endogenous non-coding RNAs involved in the regulation of mRNA stability and protein synthesis. In our research, resveratrol isolated from Vitis heyneana Roem. & Schult Vitis heyneana was observed to suppress lipopolysaccharides (LPS)-induced COX-2 expression in Raw264.7 cells in a dose dependent manner. Using qPCR it was revealed that LPS induced the expression of miR-25, miR-125a, miR-125b, miR-146a-5p, miR-146a-3p and miR-455. However, we only observed miR-146a-5p expression significantly decreased in resveratrol compared to untreated-control group. In addition, resveratrol abrogated the effect of miR-146a-5p mimic induced-COX-2 expression in Raw264.7 cells. Taken together, this study demonstrated for the first time the involvement of miR-146a-5p in resveratrol inhibited LPS-induced COX-2 expression in Raw264.7 cells.