Romanian Society of Pharmaceutical Sciences
 

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RESEARCH REGARDING TARAXACUM OFFICINALE (L.) WEBER II. OPTIMUM EXTRACTION PARAMETRES OF CAFFEIC ACID DERIVATIVES USING ULTRASOUND – ASSISTED EXTRACTION

NATELA STYLIANOU1, VASSILIS GEKAS2, ADRIANA IULIANA ANGHEL3*, VIORICA ISTUDOR1

1.University of Medicine and Pharmacy “Carol Davila”, Faculty of Pharmacy, 6 Traian Vuia, 020956, Bucharest, Romania
2 .Cyprus University of Technology, Department of Agricultural Sciences, Biotechnology and Food Science, Lemesos 3603, Cyprus
3 .University of Medicine and Pharmacy “Carol Davila”, Faculty of Pharmacy, Department of Pharmaceutical Botany, 6 Traian Vuia, 020956, Bucharest, Romania

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Pharmacological studies confirmed the therapeutic properties of the phenolic acids from plants, and their efficiency in treatment of type 2 diabetes mellitus. Based on this and also, on the facts that Taraxacum officinale L. contains a considerable amount of caffeic acid derivatives (48.8 mg/g in the leaves) and it does not exhibit a cytotoxic activity, we aimed to obtain a pharmacological active dry extract, titrated in cichoric acid, which can be developed in a pharmaceutical product used in type 2 diabetes mellitus therapy. The aim of this work was to optimize and evaluate the extraction parameters of caffeic acid derivatives from the plant material using Ultrasound - Assisted Extraction (UAE). The effects of different parameters of caffeic acid derivatives yields, in plants extracts were investigated using an experimental design - Taguchi L16 (45) orthogonal array design. An HPLC/UV method was employed for the qualitative and quantitative determination of caffeic acid derivatives from the liquid extracts and from the lyophilized extract. The amount of caffeic acid derivatives and cichoric acid in the dry extract was 127.0 mg/g and 107.50 mg/g.