Romanian Society of Pharmaceutical Sciences
 

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PROTECTIVE EFFECT OF TRIFOLIUM PRATENSE EXTRACT ON OXIDATIVE STRESS INDUCED BY BISPHENOL A IN RATS

DANIELA-SAVETA POPA1, DANIELA HANGANU2*, LAURIAN VLASE3, BÉLA KISS1, FELICIA LOGHIN1, GIANINA CRIȘAN4

1.“Iuliu Haţieganu” University of Medicine and Pharmacy Cluj-Napoca, Faculty of Pharmacy, Department of Toxicology, 8 Victor Babeş, 400012 – Cluj-Napoca, România
2.“Iuliu Haţieganu” University of Medicine and Pharmacy Cluj-Napoca, Faculty of Pharmacy, Department of Pharmacognosy, 8 Victor Babeş, 400012 – Cluj-Napoca, România,
3.“Iuliu Haţieganu” University of Medicine and Pharmacy Cluj-Napoca, Faculty of Pharmacy, Department of Pharmaceutical Technology and Biopharmacy, 8 Victor Babeş, 400012 – Cluj-Napoca, România
4.“Iuliu Haţieganu” University of Medicine and Pharmacy Cluj-Napoca, Faculty of Pharmacy, Department of Pharmaceutical Botany, 8 Victor Babeş, 400012, Cluj-Napoca, România

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Bisphenol A (BPA), a food contaminant yielded by plastic packaging, is known as xenoestrogen and oxidative stress inductor. Trifolium pratense L. (TP) is a natural source of phytoestrogens as well as of polyphenols. The aim of the present study was to evaluate the antioxidant potential and the protective effect of a TP hydroalcoholic extract in female rats subchronically exposed to BPA. Plasma samples were analysed after 30, 60 and 90 days of exposure to BPA (50 mg/kg bw) and/or a TP diluted tincture (2 mg total isoflavones / kg bw). Malondialdehyde (MDA), a lipid peroxidation end-product, and 2,3-dihydroxybenzoic acid (2,3-DHBA), a marker of in vivo hydroxyl radical production, were used to evaluate the oxidative stress generation. BPA causes the increasing of total MDA plasma levels. The simultaneous administration of TP extract countered the effect of BPA on lipid peroxidation. BPA is not a significant source of free hydroxyl radicals in vivo. However, TP extract diminished in vivo hydroxyl radical generation compared with control group (p < 0.05 after 90 days). In conclusion, TP showed a protective antioxidant effect that increased with prolonged period of repeated administration.