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PROTECTION MECHANISM OF ERYTHROPOIETIN ON CERAMIDE-INDUCED RETINAL DAMAGE

CHENG QIAN, GUANGMING WAN, WENZHAN WANG, PANSHI YAN, SHENZHI LIANG, YU ZHU*

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This study aimed to analyse the protective effect and mechanism of erythropoietin (EPO) on ceramide-induced retinal damage. First of all, ceramide was used to induce retinal damage on rats, then EPO effect on retinal damage was studied. Annexin V-FITC/PI cell apoptosis detection kit was used to detect the apoptosis rate of retinal cells (R28) and the obtained apoptosis rates of retina R28 cells were recorded in real time. Results showed that, under the effect of different concentrations of EPO, the release of lactate dehydrogenase (LDH) in the retina had an inhibiting effect on apoptosis, concentration dependent. The staining results of terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labelling (TUNEL) assay showed that, the number of TUNEL positive cells showed a significantly increasing tendency in the ganglion cell layer of retina. In addition, the apoptosis rate of nerve cells was reduced under the effect of EPO. In conclusion, EPO can protect the ceramideinduced retinal damage to a certain extent, which provides a great perspective for further studies.