Romanian Society of Pharmaceutical Sciences

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POLYGONUM BISTORTA L. HERBA ET FLORES. POLYPHENOLS PROFILE, ANTIOXIDANT PROPERTIES AND CYTOTOXIC EFFECT ON MURINE FIBROBLAST CELL LINE NIH3T3

LUCIA PÎRVU 1, FAWZIA SHA’AT 2,3*, LUMINIȚA CLAUDIA MICLEA 2, TUDOR SAVOPOL 2, GEORGETA NEAGU 1, DENISA IOANA UDEANU 4, MIHAELA GEORGETA MOISESCU 2

1.National Institute for Chemical - Pharmaceutical Research and Development (INCDCF), 112 Vitan Road, 031299, Bucharest, Romania
2.“Carol Davila” University of Medicine and Pharmacy, Faculty of Medicine, Biophysics and Cellular Biotechnology Department, 8 Eroii Sanitari Boulevard, 050474, Bucharest, Romania
3.Politehnica University of Bucharest, Faculty of Applied Chemistry and Material Science, Analytical Chemistry and Environmental Engineering Department, 1-7 Gheorghe Polizu Street, 011061, Bucharest, Romania
4.“Carol Davila” University of Medicine and Pharmacy, Faculty of Pharmacy, Department of Clinical Laboratory and Food Safety, 6 Traian Vuia Street, 020956, Bucharest, Romania

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This work aimed to study the polyphenols content, the antioxidant activity and the potential cytotoxic effects on fibroblast murine cells NIH3T3 of an ethanolic extract (70%, v/v) obtained from the aerial part (herba et flores) of Polygonum bistorta L. (Polygonaceae family). The tested extract was assessed for its total phenols content: 1.15 mg gallic acid equivalents (GAE) per 1 mL extract and showed numerous flavonoids compounds such as quercetin, kaempferol and luteolin derivates, as well as caffeic and chlorogenic phenolic acids. The chemiluminescence (CL) tests indicated a high antioxidant capacity (IC50 = 0.57 μg GAE/mL), superior to gallic acid (IC50 = 0.85 μg/mL) and rutin (IC50 = 2.54 μg/mL), used as reference compounds. The cytotoxicity test demonstrated that, at concentrations over 25 μg GAE/mL extract, the ethanolic extracts from the aerial part of Polygonum bistorta plant species have a significant toxicity on mouse embryonic fibroblast NIH3T3 cells.