Romanian Society of Pharmaceutical Sciences
 

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PHYTOCOMPONENTS IDENTIFICATION IN MISTLETOE (VISCUM ALBUM) YOUNG LEAVES AND BRANCHES, BY GC-MS AND ANTIPROLIFERATIVE EFFECT ON HEPG2 AND MCF7 CELL LINES

DALIBORCA C. VLAD1, ROXANA POPESCU2*, VICTOR DUMITRASCU1, ADINELA CIMPORESCU3, CRISTIAN S. VLAD1, CSABA VÁGVÖLGYI4, JUDITH KRISCH5, CRISTINA DEHELEAN6, FLORIN G. HORHAT7

1.Department of Biochemistry and Pharmacology, “V. Babes” University of Medicine and Pharmacy, 2 Eftimie Murgu, Timisoara, Romania
2 .Department of Cell and Molecular Biology, “V. Babes” University of Medicine and Pharmacy, 2 Eftimie Murgu, Timisoara, Romania
3 .Emergenty County Clinical Hospital, 10 Iosif Bulbuca, Timisoara, Romania
4 .Department of Microbiology, Faculty of Science and Informatics, University of Szeged, 52 Közép fasor, Szeged, Hungary
5 .Institute of Food Engineering, Faculty of Engineering, University of Szeged, 7 Mars tér, Szeged, Hungary
6 .Department of Toxicology, Faculty of Pharmacy, “V. Babes” University of Medicine and Pharmacy, 2 Eftimie Murgu, Timisoara, Romania
7 .Department of Microbiology, “V. Babes” University of Medicine and Pharmacy, 2 Eftimie Murgu, Timisoara, Romania

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In the present work, aqueous and alcoholic extracts of Viscum album young leaves and branches were evaluated, in order to identify the most relevant constituents that confirm its biological activity. GC-MS was the employed technique used for separation and identification of the extracted components. Both extracts revealed the presence of important terpenoids, fatty acids and natural antioxidants such as vitamin E. The antiproliferative potential of the obtained extracts was also investigated on neoplastic cell lines, HepG2 and MCF-7. The alcoholic extract was more efficient and proved a significant cytotoxic effect at a concentration of 75 mg/mL, but inhibition of cell proliferation was also observed when the aqueous extract was used.