Romanian Society of Pharmaceutical Sciences
 

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OPTIMIZATION AND INFLUENCE OF SOME PHYSICO-CHEMICAL PARAMETERS ON THE SYNTHESIS OF CHITOSAN-TRIPOLYPHOSPHATE NANOPARTICLES

IOANA-MIRELA VASINCU 1#, LENUȚA PROFIRE 1, MARIA APOTROSOAEI 1, ANCA ROXANA PETROVICI 2#, MARIANA PINTEALĂ 2, ALEXANDRA JITĂREANU 3*, SANDRA-MĂDĂLINA CONSTANTIN 1

1.Department of Pharmaceutical Chemistry, Faculty of Pharmacy, “Grigore T. Popa” University of Medicine and Pharmacy, 16 Universității Street, 700115, Iaşi, Romania
2.Centre of Advanced Research in Bionanoconjugates and Biopolymers Department, “Petru Poni” Institute of Macromolecular Chemistry, 41A Aleea Grigore Ghica-Voda, 700487, Iaşi, Romania
3.Department of Toxicology, Faculty of Pharmacy, “Grigore T. Popa” University of Medicine and Pharmacy, 16 Universităţii Street, 700115, Iaşi, Romania

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In the past decades, the researchers have focused their attention on the synthesis of new polymeric nanoparticles as drug delivery systems based on their small particle size and large surface area. The aim of this study was to optimize the synthesis of chitosan-based nanoparticles (CSNPs) and to study the influence of different parameters on the formulation process. The CSNPs were prepared by the ionic gelation method using sodium tripolyphosphate (TPP) as cross-linking agent and two types of chitosan (CS) (low and medium molecular weight). In order to optimize the preparation method, several parameters, such as the CS and TPP concentration, the pH of CS solution, the stirring speed and the reticulation time have been varied. The CSNPs synthesis was monitored by dynamic light scattering (DLS) technique. The results showed that the optimized method use 0.1% TPP and 0.1% CS low molecular weight in ratio of 3:1, with pH ranking between 4.7 and 4.8 and stirring at 1000 rpm for 1 h. In these conditions the size of CSNPs was 208.8 ± 8.5 nm with 0.151 polydispersity index. Based on the obtained results it could be appreciated that optimized CSNPs could be further used as drug carriers.