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MYRICETIN INHIBITION ON MURINE GLIOMA GL261 CELL LINE

HAIDONG GONG, HONGZHE LI, JINKU LI, CHUANG WANG*

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This study aimed to investigate and compare myricetin activity on mouse glioma GL261 cell line such as inhibition of the cytoactivity, block of cell proliferation and induction of GL261 apoptosis. Two samples, namely myricetin and myricetin plus myricetrin, were used to compare the effect on cell proliferation after 24 h, 48 h and 72 h respectively. Cytoactivity of GL261 cells was inhibited by myricetin in concentration of 25 μM. The inhibition of cytoactivity was more obvious when concentration increased and the action time prolonged. When myricetin was applied in G1 stage, the cell cycle was blocked. We noticed that the number of surviving GL261 cells was reduced by myricetin and apoptosis occurred. The association between myricetin and myricetrin had a synergistic inhibitory effect on cell proliferation that was superior compared with myricetin alone. Regarding the cells survival we observed that the apoptosis of GL261 cells was related to the dose of myricetin and myricetrin and also to the ratio of their combination.