Romanian Society of Pharmaceutical Sciences
 

« Back to Farmacia Journal 4/2013

MURINE STUDIES REGARDING THE VARIATION OF OXIDATIVE STATUS IN SERUM, HEPATIC AND BRAIN SAMPLES, AFTER ADMINISTRATION OF SOME CNS ACTIVE DRUGS

ALINA CRENGUŢA NICOLAE1, NICULINA MITREA1, CRISTINA MANUELA DRĂGOI1*, MARIA ZINAIDA CONSTANTINESCU1, CRISTINA CIOFRÂNGEANU2, GHEORGHE BĂRBOI3, ANDREEA LETIŢIA ARSENE1

1.Department of Biochemistry, Faculty of Pharmacy, University of Medicine and Pharmacy ”Carol Davila”, 6, Traian Vuia Str, sector 2, Bucharest, Romania
2.Faculty of Biology, University of Bucharest, 91-95, Splaiul Independenţei Str, Sector 5, Bucharest, Romania
3.Virology Department, Institute for Diagnosis and Animal Health, 63 Dr. Staicovici Str, sector 5, Bucharest, Romania

Download Full Article PDF

The imbalance between the production and accumulation of reactive oxygen species and the body’s ability to manage them by exogenous and endogenous antioxidant defense systems leads to oxidative stress. An important source to generate reactive oxygen species (ROS), as superoxide anions (O2 •-) and hydrogen peroxide (H2O2) is the mitochondrial and microsomal system. The present study assessed the variation of oxidative status in mice, after the intraperitoneal administration of some CNS active drugs. Serum, hepatic and brain samples were used throughout the study. A chemiluminescent method was applied in order to evaluate the oxidative status. Despite the fact that the brain is considered highly sensitive to oxidative damage, explained by the large amount of readily oxidizable fatty acids and poor antioxidant defenses, in our study, for cerebral and hepatic tissues we proved that the administration of tested drugs led to a decrease of the oxidative status, while in the case of serum it was developed an increase of ROS concentrations. The oxidative stress attack limitation using therapeutic agents with additional antioxidant effect, could be a therapeutic option leading to reduced doses and adverse effects of some CNS active drugs.