Romanian Society of Pharmaceutical Sciences

« Back to Farmacia Journal 6/2014

MONITORING OF SKIN PENETRATION AND ABSORPTION WITH A NEW IN VIVO EXPERIMENTAL MODEL

SZILVIA BERKÓ1, BOGLÁRKA BALÁZS1, BLANKA SÜTŐ1, GÁBOR ERŐS2, BRIGITTA GÁL2, ANITA SZTOJKOV-IVANOV3, CODRUȚA SOICA4, PIROSKA SZABÓ-RÉVÉSZ1, LAJOS KEMÉNY2, ISTVÁN ZUPKÓ3, ERZSÉBET CSÁNYI1*

1.Department of Pharmaceutical Technology, University of Szeged, 6720 Szeged, Eötvös street 6., Hungary
2.Department of Dermatology and Allergology, University of Szeged, 6720 Szeged, Korányi street 6-8., Hungary
3.Department of Pharmacodynamics and Biopharmacy, University of Szeged, 6720 Szeged, Eötvös street 6., Hungary
4.Department of Pharmaceutical Chemistry, University of Medicine and Pharmacy "Victor Babes" Timisoara, 300041 Timisoara, 2 Eftimie Murgu Str., Romania

Download Full Article PDF

The purpose of the study was to use a new type of in vivo animal model developed by our research group for the investigation of skin penetration, compared to the results of Franz cell measurements. The new in vivo experimental model provides possibility for the exact measurement of the quantity of the penetrated drug through the living mouse skin. Two gel formulations containing ibuprofen were investigated with or without penetration enhancer. The applied penetration enhancer was Transcutol®. The effect of the penetration enhancer was clearly detected in both models. The in vitro diffused amount of ibuprofen was significantly higher than the one permeated through the living skin. The presence of the active agent in the plasma was also determined after the observation period. It was possible to monitor the penetration and absorption of topical formulations simultaneously.