MODELLING OF TRANSFER KINETICS OF TRICHLORPHON PESTICIDE ACROSS CUPROPHANE DIALYSIS MEMBRANE IN PRESENCE AND ABSENCE OF ANTIDOTE POWDERS
GHEORGHE CORLAN, GABRIELA PAHOMI, ROXANA SANDULOVICI, IRINA PRASACU, MIHAELA IONESCU, VALENTINA ANUTA*
1”Carol Davila” University of Medicine and Pharmacy, 37 Dionisie Lupu St., Bucharest, Romania
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The objectives of the paper were to analyse and to model the data concerning
diffusion through a membrane with or without adsorptive powders of a hydrophilic
organophosphorous pesticide, Trichlorphon (TCP), using in vitro experimental models and
to estimate the antidote efficacy of an adsorptive powder.
Data concerned the in vitro transfer across a dialysis membrane using a static
Franz diffusion cell battery and a flow through cell system. A mixture of cellulose acetate
powder and bentonite powder was applied on the membrane in order to reduce the transfer
of toxics across membrane.
As mathematical models, linear biphasic regression was tested, first order
kinetics and a square root law derived from diffusion equation. Fitting of experimental data
with theoretical models was performed using least square methods. For selecting one or two
phase linear regression models it was used the F-test.
The release kinetics was biphasic: a first model for 50 – 60 % of the transfer of
available fraction of toxic, followed by a more or less saturation phase. Adsorptive powder
applied as antidote candidate significantly reduced the amount of transferred Triclorfon
across membrane – at least for some hours, suggesting a possible similar effect at the level
of intact skin. Presence of powders changed the mathematical transfer model from a model
dominated by a first order kinetic to a square root model.