Romanian Society of Pharmaceutical Sciences
 

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INVESTIGATION OF CLASS 1 INTEGRONS WITH ANTIBIOTIC RESISTANCE GENES IN MULTIDRUG-RESISTANT ACINETOBACTER BAUMANNII STRAINS AND DETERMINATION OF PLANT EXTRACT EFFECTS ON MULTIDRUG-RESISTANT ISOLATES

AYŞEGÜL SARAL 1, SEDANUR CINEMRE 2, FUNDA OKUMUŞ 2, ZEYNEP AKAR 3, AYŞEGÜL ÇOPUR ÇIÇEK 4, AZER ÖZAD DÜZGÜN 3*

1Department of Nutrition and Dietetics, Faculty of Health Sciences, Artvin Coruh University, Artvin, Turkey
2 Department of Biotechnology, Institute of Natural Sciences, Gumushane University, Gümüşhane, Turkey
3 Department of Genetics and Bioengineering, Faculty of Engineering and Natural Sciences, Gumushane University,
Gümüşhane, Turkey
4 Recep Tayyip Erdoğan University, Faculty of Medicine, Department of Medical Microbiology, Rize, Turkey

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This study aimed to investigate the presence of resistance genes in multidrug-resistant A. baumannii isolates as well as to determine the antibacterial activity of selected plant extracts against isolates. 41 strains were isolated from various clinical samples. PCR tests were performed using the primers. Methanol was used as solvent for the preparation of the plant extracts. MIC values of the plant extracts were determined by the broth microdilution method. The blaOXA-23, blaCTX-M-1, blaCTX-M-2, blaGES genes and Class 1 integrons were detected in five isolated strains. The lowest MIC value (2.25 mg/mL) was determined for the Echinacea purpurea extract, while the highest MIC value (50 mg/mL) was determined for the Morus alba extract. Determination of the antibacterial effect of plants extracts used in the study against A. baumannii isolates shows the importance of screening the antibacterial activity of plants in the fight against antibiotic resistance.