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IN VITRO ANTIMICROBIAL, ANTIBIOFILM AND CYTOTOXIC ACTIVITIES OF THE EXTRACTS OF ARUM ITALICUM MILLER LEAVES

MAYRAM HACIOGLU 1, BUSRA KULAKSIZ 2, KERIM ALPINAR 3, EBRU HACIOSMANOGLU 4, A. SEHER BIRTEKSOZ TAN 1*

1Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Istanbul University, Beyazit, Istanbul, 34116, Turkey
2 Department of Pharmacognosy, Faculty of Pharmacy, Biruni University, Zeytinburnu, Istanbul, 34010, Turkey
3 Department of Pharmaceutical Botany, Faculty of Pharmacy, Biruni University, Zeytinburnu, Istanbul, 34010, Turkey
4 Department of Biophysics, Faculty of Medicine, Bezmialem Vakıf University, Fatih, İstanbul, 34093, Turkey

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In this study, the antimicrobial, antibiofilm and cytotoxic activities of four different extracts which were prepared from the leaves of Arum italicum Miller, were investigated against various pathogenic microorganisms. The minimum inhibitory concentration (MIC) values of the extracts were determined by a microdilution method according to CLSI criteria. Inhibition of biofilm adhesion and biofilm formation tests were performed for extracts showing antimicrobial activity. In addition, the cytotoxic activities and anti-proliferative effects of the extracts were examined by the MTT viability test on the human umbilical vein endothelial cell line (HUVECs) and human breast cancer line (MDA-MB-231). The obtained results suggest that A. italicum extracts showed antimicrobial activity and were effective for inhibiting the pre-formed biofilms. In addition, the extracts significantly reduced the cell viability in the breast cancer cell line. According to our results, A. italicum extracts can be used as antimicrobial, antibiofilm and anticancer agents.