Romanian Society of Pharmaceutical Sciences
 

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IN VITRO AND IN VIVO APPROACH FOR THE DETERMINATION OF THE ANTI-ARTHRITIC POTENTIAL OF PSEUDOEPHEDRINE

HASEEB AHSAN 1*, HAFIZ MUHAMMAD IRFAN 1, ALAMGEER 2, ZAHID IMRAN 1, MUHAMMAD SHAFEEQ-UR- RAHMAN 3, HUMAYUN RIAZ 4

1Faculty of Pharmacy, College of Pharmacy, University of Sargodha, Pakistan
2 University College of Pharmacy, University of Punjab, Lahore, Pakistan
3 Faculty of Pharmacy, University of Central Punjab, Lahore, Pakistan
4 Rashid Latif College of Pharmacy, Rashid Latif Khan University, Lahore, Pakistan

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Based on the traditional and scientific value of Ephedra against rheumatoid arthritis, the current study was projected to evaluate the therapeutic value of the main constituent of this plant. Pseudoephedrine, the active ingredient of Ephedra, has been tested in this study by using in vitro and in vivo techniques. For the in vitro study, various concentrations (50 - 6400 µg/mL) of pseudoephedrine were used to evaluate the effect on human red blood cells membrane and to establish the inhibitory effect on protein destruction. In vivo studies were carried out using egg albumin-induced paw oedema and formaldehydeinduced arthritis model. The results demonstrated a dose dependent protection on egg and bovine serum albumin denaturation. The tested drug showed a maximum membrane stabilizing effect at a dose of 6400 μg/mL and a pharmacologic effect at a dose of 40 mg/kg bw.