Romanian Society of Pharmaceutical Sciences
 

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EXPERIMENTAL PHARMACOLOGICAL MODEL OF DIABETES INDUCTION WITH ALOXAN IN RAT

SIMONA NEGREŞ1, CORNEL CHIRIŢĂ1*, ELENA MOROŞAN2, ANDREEA LETIŢIA ARSENE3

1Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, University of Medicine and Pharmacy “Carol Davila”, Bucharest, Romania
2Department of Clinical Laboratory and Nutritional Hygiene, Faculty of Pharmacy, University of Medicine and Pharmacy “Carol Davila”, Bucharest, Romania
3Department of Biochemistry, Faculty of Pharmacy, University of Medicine and Pharmacy “Carol Davila”, Bucharest

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An experimental pharmacological model of diabetes induced with aloxan in white, male and female Wistar rats was designed. A collectivity of 312 animals was used, with an initial mean weight of 225.1 ± 31.48 g-bw and basal mean glycaemia of 103.0 ± 34.22 mg/dL. Diabetes was induced by administering intraperitoneally a dose of 130 mg/kg-bw alloxan. Experimental results showed that, in the tested collectivity, 66.34% of animals became diabetic, with a mean glycaemic value of 450.4 ± 129.7 mg/dL. For the diabetic group, decreases in the enzymatic activity were registered for glucose-6-phosphate dehydrogenase and hexokinase, accompanied by increases in the activity of glucose-6- phosphatase, as evidentiated in the experimental model of streptozotocin-induced diabetes. The alloxanic diabetes induced significant alterations of lipidic profiles in rat compared to the non-diabetic group, as follows: total cholesterol increased by 51.19%, plasma triglycerids increased by 50.30% and LDL-cholesterol increased by 59.46%. The designed pharmacological model can assist further in determining the antidiabetic potential of newly synthesized compounds or plant extracts.