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EXPERIMENTAL AND MOLECULAR MODELING INVESTIGATION OF THE REACTION BETWEEN CEFTRIAXONE AND SODIUM NITRITE. POTENTIAL MEDICAL IMPLICATIONS

MARINELA FLOREA ^#*, CORINA CRISTINA ARAMĂ ^#, ANGELA NEDELCU ^#, IOANA CLEMENTINA CONSTANTINESCU ^#

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The paper presents a new spectrophotometric method for the assay of ceftriaxone as diazonium salt and the potential medical implications of the reaction with sodium nitrite. Using KBr as catalyst, at 0 - 5°C and in acidic media a soluble and stable compound with an absorption maximum at 504 nm was obtained. The method was linear in the range 85 - 171 µg/mL, with limits of detection and quantification of 2.52 µg/mL and 7.65 µg/mL, respectively. For the diazonium ion, molecular modelling indicated as stabilization mechanism an internal charge transfer and resonance light scattering spectra confirmed it. The method is simple, rapid, accurate and precise, and successfully applied for the determination of ceftriaxone in pharmaceutical products. Ceftriaxone administration can lead to nephrolithiasis, the high urinary antibiotic and calcium concentrations being indicated to be the risk factors. The results of this study also indicated that the reaction of ceftriaxone with sodium nitrite could also have potential medical implications in this regard.