Romanian Society of Pharmaceutical Sciences
 

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EVALUATION OF THE POSSIBLE ENDOCRINE DISRUPTIVE EFFECT OF BUTYLATED HYDROXYANISOLE, BUTYLATED HYDROXYTOLUENE AND PROPYL GALLATE IN IMMATURE FEMALE RATS

ANCA POP1, CRISTIAN BERCE2, POMPEI BOLFA3*, ANDRAS NAGY3, CORNEL CATOI3, ION-BOGDAN DUMITRESCU4, LUMINITA SILAGHI-DUMITRESCU5, FELICIA LOGHIN1

1IuliuHaţieganu University of Medicine and Pharmacy, Faculty of Pharmacy, Department of Toxicology, Cluj-Napoca, Romania
2University of Agricultural Sciences and Veterinary Medicine, Faculty of Veterinary Medicine, Department of Animal Reproduction, Gynecology and Obstetrics, Cluj-Napoca, Romania
3University of Agriculture Sciences and Veterinary Medicine, Faculty of Veterinary Medicine, Department of Pathology, Cluj-Napoca, Romania
4Carol Davila University of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Physics and Informatics, Bucharest, Romania
5Babes-Bolyai University, Faculty of Chemistry and Chemical Engineering, Department of Organic Chemistry, Cluj-Napoca, Romania

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We evaluated the possible endocrine disruptive effect of butylated hydroxyanisole (BHA) (300mg/kg bw), butylated hydroxytoluene (BHT) (75mg/kg bw) and propyl gallate (PG) (405mg/kg bw), compounds extensively used as antioxidants in foods, food packaging, cosmetics and pharmaceuticals using the immature rat uterotrophic assay. To investigate their estrogenic and/or antiestrogenic effect we used 17-21 days old Wistar female rats that were given suspensions of the studied compounds for three consecutive days. Absolute and relative uterus weights were significantly decreased by all three compounds, while endometrial epithelium thickness was significantly affected only by propyl gallate when compared to the negative control. The data obtained from the present study suggests that BHA, BHT and PG indeed have endocrine disruptive effects on Wistar prepubescent female rats, the most aggressive compound being PG.