Romanian Society of Pharmaceutical Sciences
 

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EVALUATION OF POLYPHENOLIC PROFILE AND ANTIOXIDANT ACTIVITY FOR CYTISUS NIGRICANS AND CYTISUS ALBUS

DANIELA HANGANU1, LORENA FILIP1, NELI-KINGA OLAH2, ANDREI MOCAN1, LAURIAN VLASE1*, OANA RAITA3, ILIOARA ONIGA1, DANIELA BENEDEC1

1.Faculty of Pharmacy, “Iuliu Haţieganu” University of Medicine and Pharmacy, 12 I. Creangă Street, 400010, Cluj-Napoca,
Romania
2.Faculty of Pharmacy, “Vasile Goldiș” West University of Arad, 86 L. Rebreanu Street, Arad, Romania; SC PlantExtrakt SRL, Rădaia, Jud. Cluj, Romania
3.National Institute for Research and Development of Isotopic and Molecular Technologies, 1 Donath Street, Cluj-Napoca, Romania

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The aim of this study was to assess the polyphenolic composition and antioxidant capacity of Cytisus nigricans L. (lack broom) and Cytisus albus Link (white broom) (Fabaceae). A qualitative and quantitative characterization of the main phenolic compounds from ethanolic extracts of aerial parts was carried out by a HPLC/UV/MS method. The total polyphenolic, flavonoids and caffeic acid derivatives content were measured by spectrophotometric methods. The in vitro antioxidant activity of these extracts was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH), Trolox (6-hydroxy-2,5,7,8- tetramethylchroman-2-carboxylic acid) equivalent antioxidant capacity (TEAC) and an electron paramagnetic resonance (EPR) based methods. The polyphenolic profile of these species revealed the common components (gentisic acid, isoquercitrin, rutin, quercitrin, luteolin, apigenin) and specific other compounds (p-coumaric acid, ferulic acid, kaempferol) for C. nigricans. The highest antioxidant capacity and total polyphenolic content were revealed by C. nigricans. Both species contain significant amounts of polyphenolic compounds and they may be considered an important source of natural antioxidants for pharmaceutical uses.