Romanian Society of Pharmaceutical Sciences
 

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EFFECTS OF GINSENOSIDE Rg3 ON THE PROLIFERATION OF GLIOMA CELLS AND NF-κB SIGNALLING PATHWAY

YINDONG MU 1, YANG LIU 2, YANKUN HAO 3, LEI YAN 1, JUN LIANG 4, JIANJIANG DONG 1*

1.Department of Histology and Embryology, Mudanjiang Medical University, Mudanjiang 157011, China
2.Office of Educational Administration, Mudanjiang Medical University, Mudanjiang 157011, China
3.Department of Medical Function, Mudanjiang Medical University, Mudanjiang 157011, China
4.Stem Cell Institute, Mudanjiang Medical University, Mudanjiang 157011, China

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The objective of this study was to assess the effects of Ginsenoside Rg3 on the proliferation of glioma cells and NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) signalling pathway and to provide a reliable reference for clinical treatments. The glioma U251 cells were cultured and treated with Ginsenoside Rg3 at different concentrations (0, 20, 40, 80, and 160 μg/mL) for 24, 48, and 72 h, respectively. The morphological changes of U251 cells were observed before and after treatment with Ginsenoside Rg3. Cell proliferation was detected by the MTT assay. The expression of P65 gene promoter was detected by Dual-Luciferase Reporter Assay. Besides, the expressions of p65 and inhibitory kappa B (IκB) were detected by Western blotting. U251 cells before Ginsenoside Rg3 treatment were structurally intact and after the treatment started to present cell shrinkage and cellular debris that become more pronounced with the increase of the treatment duration. The lower concentration of Ginsenoside Rg3 could promote the expression of the P65 gene promoter, while the expression gradually decreased with the increase of Ginsenoside Rg3 concentration. The expression of IκBα protein gradually increased with the increase of Ginsenoside Rg3 concentration. In conclusion, Ginsenoside Rg3 can effectively inhibit the proliferative activity of U251 cells and the NF-κB signalling pathway and induce the apoptosis of tumour cells.