Romanian Society of Pharmaceutical Sciences

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EFFECT OF VITAMIN D ON CARBONIC ANHYDRASE ACTIVITY EXPERIMENTAL REASEARCH IN VITRO AND IN VIVO

CAMELIA ELIZA MRAZ1*, MARIANA MUREŞAN1, OTILIA MICLE1, LAURA VICAŞ2, ANNAMARIA PALLAG2, MARCELA COLTĂU3, IOAN PUŞCAŞ3

1University of Oradea, Medicine and Pharmacy Faculty, Preclinical II Department, Str. 1 Decembrie, Nr. 10, Oradea, Romania
2University of Oradea, Medicine and Pharmacy Faculty, Department of Pharmacy, Str. 1 Decembrie, Nr. 10, Oradea, Romania
3City Hospital "Prof.Dr.Ioan Puşcaş" Research and Nursing Center, Şimleu Silvaniei, Sălaj, Romania

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The purpose of this study was to determine the effect of vitamin D on the activity of erythrocyte carbonic anhydrase isoenzymes (CA) in vitro and in vivo. In vitro, the effect was assessed by purified CA I and CA II isoenzymes using vitamin solutions with concentrations between 10-8 and 10-4 M, and in vivo, in mice erythrocytes from Male Sprague-Dawley breed. Measurement of enzyme activity was performed by the hydration reaction of CO2 (Stopped-flow method). In vitro there is a direct inhibition of CA by a dose-response relationship maximum at concentration 10-4 M. In vivo, the inhibitory effect is stronger for vitamin D3. Both in vitro and in vivo, vitamins D2 and D3 produced a significant decrease in CA II activity compared to CA I.