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DOSAGE OF STREPTOZOTOCIN FOR INDUCING MODELS OF DIABETES MELLITUS IN OBESITY RATS WITH ENDOTHELIAL DYSFUNCTION

ANDREEA CLIM 1#, MINELA AIDA MARANDUCĂ 1,2#, ALIN CONSTANTIN PINZARIU 1, IONUT FLAVIU FAUR 3,4, BOGDAN HUZUM 5, MONICA HĂNCIANU 6*, ANDREI SZILAGYI 7, BOGDAN IONEL TAMBA 7, DRAGOMIR NICOLAE ȘERBAN 1, IONELA LĂCRĂMIOARA ȘERBAN 1

1Department of Morpho-Functional Sciences II, Discipline of Physiology, “Grigore T. Popa” University of Medicine and Pharmacy, 700115 Iași, Romania
2 Internal Medicine Clinic, “St. Spiridon” County Clinical Emergency Hospital, 700115 Iași, Romania
3 IInd Surgery Clinic, Timișoara Emergency County Hospital, 300723 Timișoara, Romania
4 Xth Department of General Surgery, “Victor Babes” University of Medicine and Pharmacy Timișoara, 300041 Timișoara, Romania
5 Department of Orthopaedic and Traumatology, Faculty of Medicine, “Grigore T. Popa” University of Medicine and Pharmacy, 16 Universitatii Street, 700115, Iași, Romania
6 ”Grigore T. Popa” University of Medicine and Pharmacy, Faculty of Pharmacy, 16 Universitatii Street, 700115, Iasi, Romania
7 Advanced Research and Development Centre in Experimental Medicine (CEMEX), Grigore T. Popa University of Medicine and Pharmacy, 700115 Iași, Romania

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This experimental research evaluated the influence and dosage of streptozotocin (STZ) in determining type 2 diabetes mellitus in an experimental model of obese rats. 62 male obese Wistar rats, aged 4 - 6 months (450 - 550 g), consumed exclusively Hight-Fat-Diet (HFD) with 2% cholesterol for 2 months were included in the present experiment in two groups, First group- control (n = 7), group II (n = 55). After 2 months, group II was administrated 35 STZ mg/kg STZ intraperitoneally (i.p) to induce Diabetes. We evaluated the level of glycemia at 7, 10 and 14 days after administration of the treatment. Also, before and after the experiment, the serum lipid profile was evaluated. Rats with blood glucose levels of 200 mg/dL or higher were considered diabetic. In our study, the dosage of 35 mg/kg of STZ in obese rats determined in the first 5 days mortality of 13 rats (23.63%) with high glucose levels (glucose level > 600 mg/dL). At 7 days, DZ was confirmed at 35 rats, and a success rate of 83.33%. Also, at 10 days, 7 rats had high values of glycemia but did not confirm DZ (16.66%). Therefore at 14 days after the STZ was administrated, 4 rats from 7 (9.52%) were confirmed with DZ. STZ is an important chemical agent used for the induction of Diabetes mellitus in laboratory animals. Age, weight and dose of Streptozotocin are important factors in mortality and success rate in inducing experimental models of type 2 Diabetes mellitus in obese rats.