Romanian Society of Pharmaceutical Sciences
 

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DNA CLEAVAGE STUDY AND SOD-MIMETIC ACTIVITY OF A NEW Cu(II) COMPLEX

ADRIANA CORINA HANGAN1, ROXANA LIANA STAN2*, BOGDAN SEVASTRE3, SÎNZIANA GHEORGHE-CETEAN1, LUMINIȚA OPREAN1

1.“Iuliu Hațieganu” University of Medicine and Pharmacy, Faculty of Pharmacy, Department of Inorganic Chemistry, 12 Ion Creangă Street, 400010, Cluj-Napoca, Romania
2.“Iuliu Hațieganu” University of Medicine and Pharmacy, Faculty of Pharmacy Department of Pharmaceutical Biochemistry and Clinical Laboratory, 6 Pasteur Street, 400012, Cluj-Napoca, Romania
3.University of Agricultural Science and Veterinary Medicine, Faculty of Veterinary Medicine, Paraclinic Department, 3-5 Calea Mănăștur Street, 400372, Cluj-Napoca, Romania

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The DNA cleavage capacity of a new Cu(II) complex with substituted N-sulphonamide ligand, [Cu(L)2(phen)(H2O)] (HL = N-(5-ethyl-[1,3,4] thiadiazole-2-yl)-benzenesulphonamide) was studied by analysing the process of damaging the DNA molecule (pUC18 plasmid) in the presence of a reducing agent. Also, we tried to elucidate the reaction mechanism through which the complex damages the DNA molecule by studying the free radicals and ions involved in the degradation of the nucleic acid structure. The use of ROS (reactive oxygen species) scavengers indicates that the hydroxyl radicals and the superoxide radical anions are the main species capable of breaking the DNA strands by an oxidative mechanism. The SOD-like activity of the complex was tested by an indirect method using the xanthine/xanthine oxidase system as source for superoxide radicals. By this way, we demonstrated the SOD mimetic activity of the complex.