DETERMINATION OF ROSMARINIC ACID BY HPTLC-IMAGE ANALYSIS IN MEDICINAL TEAS AND THEIR BIOLOGICAL PROPERTIES
DANIELA BENEDEC #, ILIOARA ONIGA*, ANNAMARIA KOZMA-IMRE, DANIELA HANGANU, VIORICA ȚĂRMURE #, EDE BODOKI
“Iuliu Hațieganu” University of Medicine and Pharmacy, 8 V. Babeș Street, Cluj-Napoca, Romania
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The aim of this study was to evaluate the rosmarinic acid (RA) content and the antioxidant and antimicrobial activity of Ocimum basilicum, Origanum vulgare, Hyssopus officinalis, Melissa officinalis, Mentha piperita, Salvia officinalis, Satureja hortensis, Rosmarinus officinalis and Thymus vulgaris available in Romanian pharmacies as herbal tea products. RA analysis was performed by HPTLC-image analysis detection. UV-VIS spectrophotometry was used for the evaluation of the polyphenolic content. The extracts were screened for antioxidant activity using the DPPH radical scavenging assay. The antimicrobial test was performed by the disk diffusion assay. The RA content of nine species, as determined by the HPTLC-image analysis, varied between 9.16 mg/g (in O. vulgare) and 2.39 mg/g (in T. vulgaris). M. officinalis and O. vulgare extracts exhibited the highest antioxidant capacity, whereas the hyssop the lowest activity, in line with their polyphenolic content. The antimicrobial test revealed an important inhibitory activity against S. aureus, for the rosemary extract. The obtained results about the RA and polyphenolic contents and antioxidant and antimicrobial activities provide necessary information in order to safe and effective use of these medicinal plants.