Romanian Society of Pharmaceutical Sciences
 

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DENATONIUM BENZOATE ATTENUATES INFLAMMATION AND PAIN AND DECREASES PGE2 LEVELS IN RATS

VESELA YULIEVA KOKOVA 1, MILENA NENKOVA DRAGANOVA-FILIPOVA 2,3, PLAMEN
IVANOV ZAGORCHEV 3,4, LYUDMIL PEYCHEV PEYCHEV 1, ELISAVETA GEORGIEVA APOSTOLOVA 1*

1Department of Pharmacology, Toxicology and Pharmacotherapy, Faculty of Pharmacy, Medical University-Plovdiv, 15A Vassil Aprilov Boulevard, 4002 Plovdiv, Bulgaria
2Department of Medical Biology, Medical Faculty, Medical University-Plovdiv, 15A Vassil Aprilov Boulevard, 4002 Plovdiv, Bulgaria
3Research Institute at Medical University-Plovdiv, 15A Vassil Aprilov Boulevard, 4002 Plovdiv, Bulgaria
4Department of Medical Physics and Biophysics, Faculty of Pharmacy, Medical University-Plovdiv, 15A Vassil Aprilov Boulevard, 4002 Plovdiv, Bulgaria

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Denatonium benzoate (DB) is an agonist of bitter taste receptors (TAS2Rs). TAS2Rs are detected in many non-sensory tissues, and their activation could lead to different effects. The aim of our study was to evaluate the anti-inflammatory, antihyperalgesic activity of DB and the changes in plasma levels of PGE2 after administration of DB. Male Wistar rats were divided into 5 groups (n = 6) and treated intraperitoneally (i.p.) with: saline, diclofenac sodium 25 mg/kg bw, DB 10 mg/kg bw and DB 15 mg/kg bw, respectively. The following methods were applied: i) carrageenan-induced paw oedema; ii) carrageenaninduced hyperalgesia and iii) enzyme-linked immunosorbent assay (ELISA). DB in doses of 10 and 15 mg/kg bw significantly inhibited paw oedema at the 2nd, 3rd and 4th hour after carrageenan injection. The anti-hyperalgesic activity was observed in rats treated with 10 and 15 mg/kg bw DB only at the 4th hour after carrageenan administration. The levels of PGE2 significantly decreased in groups treated with DB in both doses. Our results indicate that DB possesses anti-inflammatory and anti-hyperalgesic effects in the carrageenan-induced models of exudative inflammation and hyperalgesia. This activity may be related to decreased PGE2 levels.