Romanian Society of Pharmaceutical Sciences
 

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CHIRAL SEPARATION OF 16 BETA-BLOCKERS ON IMMOBILIZED POLYSACCHARIDE CHIRAL STATIONARY PHASES

RADU-CRISTIAN MOLDOVAN1, GABRIEL-SORIN DASCĂL1, VALENTIN MIREL2, EDE BODOKI1*, RADU OPREAN1

1.Department of Analytical Chemistry and Instrumental Analysis, Faculty of Pharmacy, “Iuliu Hațieganu” University of Medicine and Pharmacy Cluj-Napoca, 4 Louis Pasteur Street, 400349 Cluj-Napoca, Romania
2.National Institute for Research and Development of Isotopic and Molecular Technologies, 67-103 Donat Street, 400293 Cluj-Napoca, Romania

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Chiral separations method development is a tedious process, involving the use of high quantities of solvents and it is aquired over a long period of time. In order to simplify the process and reduce the time needed for selecting the optimal stationary phase for separating the enantiomers of interest, a normal phase gradient elution was developed using n-hexane/2-propanol as mobile phase with an alcohol gradient ranging from 20% to 50% (v/v). Four commercially available polysaccharide based stationary phases were tested on a set of 16 chiral beta-blockers. The effect of three basic additives was evaluated regarding their effect on selectivity. It was observed that depending on the stationary phase, the additives can have an important influence. This approach can supply a convenient set of data for future quantitative correlations between chemical structure of enantiomers and experimental chromatographic parameters. Moreover, the optimal mobile phase composition can be chosen for further development in isocratic mode.