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CHEMICAL COMPOSITION AND ANTIOXIDANT ACTIVITY OF FICUS ELASTICA ROXB. EX HORNEM AND RAPHANUS SATIVUS L. SELECTIVE DRY EXTRACTS WITH POTENTIAL ANTIDIABETIC ACTIVITY

AL HILFI ZAID ABDULRIDHA FLAYYH 1#, IOANA NENCU 2*, TEODORA COSTEA 2#, CERASELA ELENA GÎRD 2, CRISTINA SILVIA STOICESCU 3#, ADRIANA IULIANA ANGHEL 3#, ROBERT VIOREL ANCUCEANU 3#, MIHAELA DINU 3, FLORIANA ELVIRA IONICĂ 4, OANA CRISTINA ŞEREMET 1, SIMONA NEGREŞ 1

1.Pharmacology and Clinical Pharmacy Department, Faculty of Pharmacy, “Carol Davila” University of Medicine and Pharmacy, 6 Traian Vuia Street, 020956, Bucharest, Romania
2.Pharmacognosy, Phytochemistry, Phytotherapy Department, Faculty of Pharmacy, “Carol Davila” University of Medicine and Pharmacy, 6 Traian Vuia Street, 020956, Bucharest, Romania
3.Pharmaceutical Botany Department, Faculty of Pharmacy, “Carol Davila” University of Medicine and Pharmacy, 6 Traian Vuia Street, 020956, Bucharest, Romania
4.Faculty of Pharmacy, University of Medicine and Pharmacy of Craiova, Craiova, Romania

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The aim of our study consisted in obtaining, phytochemical and antioxidant activity evaluation of two selective dry extracts from the leaves of Ficus elastica Roxb. ex Hornem and Raphanus sativus L., with potential use in metabolic diseases. The results of the phytochemical assessment of the two hydroethanolic dry extracts (the employed hydroethanolic mixture were: 70% ethanol (v/v) for Fici elastici folium and 50% ethanol (v/v) for Raphani sativi folium) indicated the following: Fici elastici extractum (FEE) - 3.6140±0.4000 g% flavonoids expressed as hyperoside, 5.950 ± 0.3800 g% phenolcarboxylic acids expressed as chlorogenic acid, 13.3299 ± 0.1583 g% total phenolic compounds expressed as tannic acid; 2. Raphani sativi extractum (RSE) - 6.7300 ± 0.6600 g% flavonoid expressed as hyperoside, 3.1263 ± 0.1500 g% phenolcarboxylic acids expressed as chlorogenic acid, 21.4175 ± 0.9454 g% total phenolic compounds expressed as tannic acid. The HPLC analysis confirmed the presence of apigenin-7-glucoside in Raphani sativi extractum. Evaluation of the antiradicalar activity of the dry extracts was determined by scavenger activity of free radicals, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2’-azinobis-(3-ethylbenzothiazoline- 6-sulfonic acid) (ABTS•+) and by ferric reducing power method. The antioxidant activity was expressed as EC50 mg/mL. The following results were obtained: EC50DPPH = 6.4166 ± 0.3329 mg/mL, EC50ABTS = 0.0768 ± 0.0020 mg/mL, EC50ferric reducing power = 0.4027 ± 0.0016 mg/mL (for FEE); EC50DPPH = 7.5100 ± 0.1414 mg/mL, EC50ABTS = 0.2611 ± 0.1893 mg/mL, EC50ferric reducing power =1.3935 ± 0.0111 mg/mL (for RSE).