ASSESSING BIOCHEMICAL AND OXIDATIVE STRESS PARAMETERS AFTER VAGINAL AND ORAL ADMINISTRATION OF 5-FLUOROURACIL IN LABORATORY ANIMALS
DANIELA-CRISTINA DIMITRIU1, CATALINA-ELENA LUPUSORU2, ILEANA COJOCARU3*, CARMEN GAFITANU3, LAURA PALADE3, RAOUL-VASILE LUPUSORU4
“Gr. T. Popa” University of Medicine and Pharmacy, Iaşi, Romania
1.Biochemistry Department
2.Pharmacology Department
3.Pharmaceutical Technology and Biopharmacy Department
4.Physiopathology Department
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Intraepithelial vaginal neoplasias represent 0.4% of the intraepithelial diseases of the lower genital tract. In order to evaluate
the advantages of vaginal administration for such disorders, we used an animal model and it was assessed: the hepatic
function, by measuring the activity of aspartate aminotransferase (ASAT), alanine aminotransferase (ALAT), the renal
function, by assessing the plasma level of the urea, creatinine and uric acid and the modification of certain oxidative stress
parameters, glutathione peroxidase (GPx), reduced glutathione (GSH) and the level of lipid peroxidation through the
malondialdehyde concentration (MDA) for a new formulation of mucobioadhesive tablets with 5-fluorouracil (5-FU) versus
the oral administration of equivalent dosages, 6 respectively 20 mg/kg body weight. The assessed parameters for the two
doses compared to control were: ASAT [UI/L] 137.71 ± 12.85 and 172.57 ± 25.47, control 103.43 ± 10.18, uric acid [mg/dL]
0.92 ± 0.09 and 2.18 ± 0.12, control 0.87 ± 0.47, Gpx [μmol GSSG/min/mL] 3.50 ± 0.63 and 4.03 ± 0.62, control 3.01 ±
0.50, GSH [μg/mL] 32 ± 2.51 and 21.5 ± 10.56, control 40 ± 5.29, MDA [μmol/mL] 0.81 ± 0.11 and 0.97 ± 0.24, control
0.50 ± 0.09. The intravaginal administration of 6 mg/kg body weight 5-FU generated small modifications at the renal and
hepatic level compared to the oral administration of the equivalent dosages and induced a smaller level of oxidative stress.
