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ANTIVIRAL ACTIVITY OF ORIGINAL FLAVONOIDS-CONTAINING PHYTOPREPARATION AGAINST HUMAN ALPHAHERPESVIRUS 2, HEPATITIS C SURROGATE VIRUS AND TRANSMISSIBLE GASTROENTERITIS CORONAVIRUS

ALEXANDER GALKIN 1, MARYNA ARKHYPOVA 1,2, DARIA STAROSYLA 2, OLEG DERIABIN 2, OLEKSANDR VASYLCHENKO 3, SVITLANA RYBALKO 2, OLENA GOLEMBIOVSKA 1*

1“Igor Sikorsky Kyiv Polytechnic Institute” National Technical University of Ukraine, Department of Translational Medical Bioengineering, Kyiv, 03057, Ukraine
2“Gromashevsky” Institute of Epidemiology and Infectious Diseases of the National Academy of Medical Sciences of Ukraine, Kyiv, 03038, Ukraine
3Institute of Molecular Biology and Genetics of the National Academy of Sciences of Ukraine, Kyiv, 03680, Ukraine

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This study evaluates the antiviral activity of a complex phytopreparation consisting of alcohol tinctures of Filipendula vulgaris, Petroselinum crispum, Apium graveolens radices, Galium verum, Linaria vulgaris herbs and Calendula officinalis flowers. The phytopreparation was tested on in vitro models of human alphaherpesvirus 2 (HHV-2), hepatitis C surrogate virus (bovine viral diarrhoea virus, BVDV) and transmissible gastroenteritis virus (TGEV). The phytopreparation solution demonstrated antiviral activity against all three viruses. The phytopreparation solution is an active inhibitor of HHV-2, BVDV and coronavirus (TGEV) replication with a selectivity index of 80, 320 and 160, respectively. Animal studies showed that the phytopreparation solution (1:100 dilution), when used as a combined treatment regimen (per os + application), delayed the onset of guinea pig genital herpes first symptoms and reduced the time of disease in vivo. The phytopreparation solution had an antiherpetic therapeutic action similar to that of acyclovir (a reference antiherpetic drug). The induction of αIFN and inhibition of RNA and DNA synthesis were suggested mechanisms of the antiviral action of the phytopreparation complex.