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ANTINOCICEPTIVE EFFECT OF 4-ALLYL-2-METHOXYPHENOL AND DICLOFENAC CO-ADMINISTRATION IN THE FORMALIN TEST IN RATS

OLGA EDITH GONZÁLEZ-LUGO 1, JUAN RAMÓN ZAPATA-MORALES 2, AMAURY DE JESÚS POZOS GUILLÉN 3, ABELARDO CAMACHO LUIS 4, PATRICIA PONCE PEÑA 5, AZAHEL DE JESÚS RANGEL LÓPEZ 1, ANGEL ANTONIO VÉRTIZ HERNÁNDEZ 1*

1Region Altiplano Academic Coordination, Autonomous University of San Luis Potosí. San Luis Potosí, México
2Pharmacy Department, Division of Natural and Exact Sciences, University of Guanajuato, México
3Faculty of Dentistry Autonomous University of San Luis Potosí. San Luis Potosí, México
4Food and Nutrition Center, Faculty of Medicine and Nutrition, Juarez University of the State of Durango, México
5Faculty of Chemical Sciences, Juarez University of the State of Durango, México

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Pain is one of the main ailments that affects humanity. To treat it, non-steroid anti-inflammatory analgesics have been widely used and also opioid analgesics, but to a lesser extent. The use of natural extracts has provided excellent results and represents a new treatment alternative. This study aimed to evaluate the antinociceptive effect of the co-administration of 4-allyl-2methoxyphenol (eugenol; clove essential oil, Syzygium aromaticum) and diclofenac through the formalin test. Eugenoldiclofenac combinations were used at total doses of 725 µg/kg b.w. and 962.5 µg/kg b.w. to measure the antinociception through the formalin test in Wistar Kyoto rats. The combination eugenol-diclofenac in a dose of 962.5 µg/kg b.w. showed the highest effect in the behavioural nociceptive inhibition compared to the combination of 725 µg/kg b.w. and individual treatments (eugenol 1200 µg/kg b.w. and diclofenac 250 µg/kg b.w.). In this context, it was obtained a 92.29 ± 0.64% of antinociception in phase 1 accentuated up to 94.84 ± 0.79% in phase 2. The eugenol and diclofenac combination in the dose of 962.5 µg/kg b.w. showed a supra additive antinociceptive effect, higher than the dose of 725 µg/kg b.w. and the individual doses, which suggests a combination of the antinociceptive mechanisms of eugenol and diclofenac.