Romanian Society of Pharmaceutical Sciences
 

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ANTIMUTAGENIC AND ANTIMICROBIAL EFFECT OF AN EXPERIMENTAL PLANT BASED EXTRACT FOR ENDODONTIC USAGE

ANDRADA VOINA-ȚONEA 1*, MĂDĂLINA VĂLEANU 2, SORANA BACIU 1, MĂDĂLINA LAZĂR 1, CORINA VOINA 1, ANDREEA MIRON 1, DAN VODNAR 3

1.“Iuliu Hațieganu” University of Medicine and Pharmacy, Faculty of Dental Medicine, Cluj-Napoca, Romania
2.“Iuliu Hațieganu” University of Medicine and Pharmacy, Faculty of Medicine, Cluj-Napoca, Romania
3.University of Agricultural Sciences and Veterinary Medicine, Faculty of Food Science and Technology, Cluj-Napoca, Romania
*corresponding author: andrada.tonea@umfcluj.ro

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An experimental extract based on Arctium lappa L. root powder and Aloe vera (L.) Burm.f. gel was developed for endodontic usage. Its effect on the microbial viability was previously tested in vitro, against bacterial and fungal strains commonly found in the human endodontic environment. The present study focuses on the in vivo evaluation of the antimicrobial effect of the experimental plant based extract, using bacteria recovered from the infected root canals of the canine species. We determined the number of colony-forming units before and after using the plant based product. The antimutagenic effect of the experimental extract was determined by detecting mutagenic compounds with the help of the Salmonella mutagenicity test (Ames test). Two auxotrophic reference strains were used, Salmonella typhimurium TA 98 and Salmonella typhimurium TA 100, which carried defective genes, related to the synthesis of histidine. Overall, results showed in vivo antimicrobial activity of the experimental extract and also antimutagenic and inhibition effect against the two mutant bacterial strains.