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ANTI-INFLAMMATORY AND ANTIOXIDANT EFFECTS OF MAHONIA AQUIFOLIUM LEAVES AND BARK EXTRACTS

ANDRA-DIANA ANDREICUŢ 1, ALINA ELENA PÂRVU 1*, AUGUSTIN CĂTĂLIN MOȚ 2, MARCEL PÂRVU 3, EVA FISCHER-FODOR 4, VASILE FELDRIHAN 5, ADRIANA FLORINELA CĂTOI 1, MIHAI CECAN 6, ALEXANDRU IRIMIE 7

1.Department of Pathophysiology, Faculty of Medicine, "Iuliu Haţieganu" University of Medicine and Pharmacy, 3-4 Victor Babeş Street, RO-400012, Cluj-Napoca, Romania
2.Department of Chemistry, Faculty of Chemistry and Chemical Engineering, "Babeş-Bolyai" University, 11 Arany Janos Street, RO-400028, Cluj-Napoca, Romania
3.Department of Biology, Faculty of Biology and Geology, "Babeş-Bolyai" University, 42 Republicii Street, RO-400015, Cluj-Napoca, Romania
4.Medfuture Research Centre for Advanced Medicine, University of Medicine and Pharmacy, “Iuliu Haţieganu”, RO-400012, Cluj-Napoca, Romania; "I. Chiricuţă" Institute of Oncology, 34-36 Republicii Street, RO-400015, Cluj-Napoca, Romania
5.Department of Imunology and Alergology, Faculty of Medicine, "Iuliu Haţieganu" University of Medicine and Pharmacy, 19-21 Croitorilor Street, RO-400162, Cluj-Napoca, Romania
6.Student, Faculty of Medicine, "Iuliu Haţieganu" University of Medicine and Pharmacy, 8 Babes Street, RO-400012, Cluj-Napoca, Romania
7.Department of Oncology, Faculty of Medicine, "Iuliu Haţieganu" University of Medicine and Pharmacy, 34-36 Republicii Street, RO-400015, Cluj-Napoca, Romania
*corresponding author: parvualinaelena@yahoo.com

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Oxidative stress and inflammation are interlinked processes that seem to play an important role in aging. The present work aimed to test the antioxidant and anti-inflammatory activity of ethanolic Mahonia aquifolium leaves and bark extracts in an experimental acute inflammation. Six polyphenols and four alkaloids were measured by HPLC. The radical scavenging activity was measured by 1,1-diphenyl-2-picrylhydrazyl (DPPH) test. Inflammation was induced in rat with turpentine oil. Anti-inflammatory activity was evaluated with serum nitric oxide (NOx) and tumour necrosis factor alpha (TNF-alpha), and oxidative stress with total oxidative status (TOS), total antioxidant reactivity (TAR), oxidative stress index (OSI), 3-nitrotyrosine (3NT), malondialdehyde (MDA) and total thiols (SH). Extracts were administrated orally (100%, 50%, 25%) for seven days prior to inflammation. The effects were compared to diclofenac. The most abundant polyphenol was chlorogenic acid, and alkaloids were identified only in the bark extract. The DPPH assay showed good results, except for the bark extract. All extracts decreased NOx, TOS, 3NT, and increased SH. TNF-alpha was reduced, and TAR was increased only by the leaves extract. MDA was not influenced. Our findings suggest that M. aquifolium leaves and bark extracts have anti-inflammatory and antioxidant effects that support the use in primary prevention of the “inflammaging” process.