ASSESSMENT OF OLIVE LEAVES EXTRACT – CYTOTOXICITY IN VITRO AND ANGIOGENESIS IN OVO
MĂDĂLINA BORUGĂ 1#, VIRGIL ENĂTESCU 1#, IULIA PÎNZARU 2*, CAMELIA SZUHANEK 3, DALIANA MINDA 2, IASMINA MARCOVICI 2, DANIELA RADU 1, DANIELA MARȚI 4, BRIGITHA VLAICU 1, OANA SUCIU 1 1“Victor Babeş” University of Medicine and Pharmacy Timișoara, Faculty of Medcine, 2 Eftimie Murgu Square, 300041, Timişoara, Romania 2“Victor Babeş” University of Medicine and Pharmacy Timișoara, Faculty of Phamacy, 2 Eftimie Murgu Square, 300041, Timişoara, Romania 3“Victor Babeş” University of Medicine and Pharmacy Timișoara, Faculty of Dental Medicine, 2 Eftimie Murgu Square, 300041, Timişoara, Romania 4Western University Vasile Goldiș, Faculty of Medicine, 94 Revoluției Boulevard, 310025, Arad, Romania *corresponding author: iuliapinzaru@umft.ro #Authors with equal contribution.
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Abstract:
In this study, it was determined the composition of the olive leaves ethanolic extract by LC-MS, the in vitro cytotoxicity on A375 human melanoma cells was established, by means of the MTT assay (number of viable cells), the Annexin V-FITC assay (number of apoptotic cells) and in vivo irritant and toxic potential by chorioallantoic membrane assay. The extract contains a number of polyphenols, among which luteolin and rutin are the predominant compounds and triterpenic acids with a significant ursolic acid content. At the highest concentration tested, 100 μg/mL, it was noted an important cytotoxic effect on human melanoma cells, it influences the development of growing capillaries, it induces narrowed calibre vessels, but no signs of toxicity on HET-CAM were recorded, while 24 h after treatment, the extract expressed a stronger effect, upon both tumour cell growth and the vascularization of A375 melanoma cells on in vivo test.

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