CYTO-HISTOPATHOLOGICAL AND PCR CORRELATION OF CATTLE PULMONARY HYDATID DISEASE
FLORICA BĂRBUCEANU 1,2*, GABRIEL PREDOI 2, CORINA-BEATRIS ANCUCEANU 1, STELIAN BĂRĂITĂREANU 2, ȘTEFANIA-FELICIA BĂRBUCEANU 3 1Institute for Diagnosis and Animal Health, 63 Dr Staicovici D. Nicolae Street, 050557, Bucharest, Romania 2University of Agricultural Science and Veterinary Medicine, Faculty of Veterinary Medicine, 105 Splaiul Independenței, 050097, Bucharest, Romania 3Department of Organic Chemistry, “Carol Davila” University of Medicine and Pharmacy, Faculty of Pharmacy, 6 Traian Vuia Street, 020956, Bucharest, Romania *corresponding author: florica.barbuceanu@idah.ro
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Abstract:
Hydatid disease is a severe zoonosis caused by metacestodes (the larval stage of a tapeworm) of the genus Echinococcus. Considering the complex ultrastructure of the cyst, the cyto-histopathological analysis of the hydatid cyst layers requires the use of various histochemical staining techniques. This paper is a cyto-histopathological and PCR analysis of the uninfected and infected pulmonary hydatid cyst membrane structures, conducted between 2009 and 2019 on a total of 2123 lung samples of cattle 2-18 years old. The open infected hydatid cyst, identified in lungs from cattle, can be considered, from the cyto-histopathological point of view, as a diffuse granulomatous hydatid proliferation. PCR was a reliable and valuable tool in the E. granulosus rapid diagnosis.

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